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Webinar: Target-enriched RNA-seq for SARS-CoV-2 surveillance and genome analysis

New SARS-CoV-2 strains are continuing to emerge, potentially impacting the effectiveness of existing vaccines. Thus, the ability to determine the genomic sequences in samples is critical for…

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Good panel design for NGS Target Enrichment

What goes into designing a target enrichment panel for targeted NGS? Creating a custom probe panel for NGS can be an efficient, cost-effective way to focus on the most relevant genomic regions while…

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Multiplexing in NGS Target Enrichment

How does multiplexing increase the efficiency of targeted NGS? This video describes how the pooling of multiple barcoded libraries enables parallel sample processing and greater sequencing…

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Low-quality samples in NGS Target Enrichment

How can high-quality NGS results be achieved with low-quality DNA samples? This video describes the challenges of working with low-quality, low-input DNA samples from a variety of sources. It also…

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On-Target Rate in NGS

What is on-target rate in next-generation sequencing (NGS), and how does it relate to the efficiency of target enrichment? This video explains the differences between % reads on-target and % bases…

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Genome Equivalent

What is a genome equivalent, and why does it matter when planning next-generation sequencing (NGS) experiments? This video describes the relationship between the number of genome equivalents in an…

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GC-Bias

What is GC-Bias in NGS, where does it come from, and how can it be reduced? This video provides an overview of GC-bias in next-generation sequencing (NGS), explaining how this metric is derived and…

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Duplication Rate in NGS

How does duplication rate affect the quality of NGS data, and what can be done to minimize duplicate reads? This video describes what duplicate reads are and how they reduce the quality of sequencing…

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Fold-80 Penalty

What is the Fold-80 base penalty score in target-enriched next-generation sequencing (NGS)? This video describes the relationship between the Fold-80 base penalty score and coverage uniformity, and…

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Webinar: RNA:Seq: Addressing the Challenges using KAPA RNA HyperPrep

RNA-sequencing (RNA-seq) and the study of transcriptomes have become essential in both molecular and clinical research. Many researchers and sequencing service providers have found RNA-seq difficult…

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11 - Elution of the DNA / libraries

Place the sample on a magnet to collect the beads. Incubate until the liquid is clear. Transfer the eluate to a new tube/well

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